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ASCC3 Polyclonal Antibody (CAB7960)

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CAB7960
  • Western blot - ASCC3 Polyclonal Antibody (CAB7960)
  • Immunofluorescence - ASCC3 Polyclonal Antibody (CAB7960)
$331.89

Description

Overview

Title:ASCC3 Polyclonal Antibody (CAB7960)
Size:100µL
Code:CAB7960
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that belongs to a family of helicases that are involved in the ATP-dependent unwinding of nucleic acid duplexes. The encoded protein is the largest subunit of the activating signal cointegrator 1 complex that is involved in DNA repair and resistance to alkylation damage. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-111 of human ASCC3 (NP_071374.1).
GeneID:10973
SwissProt:Q8N3C0
Synonyms:ASCC3; ASC1p200; HELIC1; RNAH; activating signal cointegrator 1 complex subunit 3
Calculated MW:13kDa/83kDa/251kDa
Observed MW:160kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:293T, Jurkat, HeLa, NCI-H460, Mouse liver, Mouse spleen, Mouse brain
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - ASCC3 Polyclonal Antibody (CAB7960)

Western blot analysis of extracts of various cell lines, using ASCC3 antibody (CAB7960) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - ASCC3 Polyclonal Antibody (CAB7960)

Immunofluorescence analysis of A549 cells using ASCC3 antibody (CAB7960). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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