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ARF6 Polyclonal Antibody (CAB3162)

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CAB3162
  • Western blot - ARF6 Polyclonal Antibody (CAB3162)
  • Immunohistochemistry - ARF6 Polyclonal Antibody (CAB3162)
  • Immunohistochemistry - ARF6 Polyclonal Antibody (CAB3162)
€299.00

Description

Overview

Title:ARF6 Polyclonal Antibody (CAB3162)
Size:100µL
Code:CAB3162
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

Immunogen Information

Immunogen:A synthetic peptide of human ARF6
GeneID:382
SwissProt:P62330
Synonyms:ARF6; ADP-ribosylation factor 6
Calculated MW:20kDa
Observed MW:19kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:100 - 1:500; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:A-549, MCF-7, U-251MG, HepG2, HeLa
Cellular Location:Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Endosome membrane, Golgi apparatus, Lipid-anchor, Midbody, Recycling endosome membrane, filopodium membrane

Antibody Images

Western blot - ARF6 Polyclonal Antibody (CAB3162)

Western blot analysis of extracts of various cell lines, using ARF6 antibody (CAB3162) at 1:250 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - ARF6 Polyclonal Antibody (CAB3162)

Immunohistochemistry of paraffin-embedded rat brain using ARF6 Antibody (CAB3162) at dilution of 1:100 (40x lens).

Immunohistochemistry - ARF6 Polyclonal Antibody (CAB3162)

Immunohistochemistry of paraffin-embedded rat kidney using ARF6 Antibody (CAB3162) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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