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ARF6 Polyclonal Antibody (CAB0988)

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CAB0988
  • Western blot - ARF6 Polyclonal Antibody (CAB0988)
  • Immunofluorescence - ARF6 Polyclonal Antibody (CAB0988)
€299.00

Description

Overview

Title:ARF6 Polyclonal Antibody (CAB0988)
Size:100µL
Code:CAB0988
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1).
GeneID:382
SwissProt:P62330
Synonyms:ARF6; ADP-ribosylation factor 6
Calculated MW:20kDa
Observed MW:19kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, DU145, Mouse brain, Rat brain, Rat spleen
Cellular Location:Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Endosome membrane, Golgi apparatus, Lipid-anchor, Midbody, Recycling endosome membrane, filopodium membrane

Antibody Images

Western blot - ARF6 Polyclonal Antibody (CAB0988)

Western blot analysis of extracts of various cell lines, using ARF6 antibody (CAB0988) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.

Immunofluorescence - ARF6 Polyclonal Antibody (CAB0988)

Immunofluorescence analysis of HeLa cells using ARF6 antibody (CAB0988) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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