APTX Polyclonal Antibody (CAB5364)
- SKU:
- CAB5364
Description
| Title: | APTX Polyclonal Antibody (CAB5364) | |
| Size: | 100µL | |
| Code: | CAB5364 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.
Immunogen Information
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1). |
| GeneID: | 54840 |
| SwissProt: | Q7Z2E3 |
| Synonyms: | APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; aprataxin |
| Calculated MW: | 5kDa/13kDa/19-40kDa |
| Observed MW: | 40kDa |
Applications
| Reactivity: | Human, Mouse |
| Tested Applications: | WB; IF; IP |
| Recommended Dilution: | WB 1:500 - 1:2000; IF 1:50 - 1:100; IP 1:20 - 1:50 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting Immunofluorescence Immunoprecipitation |
| Positive Samples: | 22Rv1, A-549, K-562, BT-474, U-87MG, HT-29, Raji |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus, nucleoplasm |
Antibody Images
| Western blot analysis of extracts of various cell lines, using APTX antibody (CAB5364) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
| Immunofluorescence analysis of A549 cells using APTX antibody (CAB5364). |
| Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (CAB5364). Western blot was performed from the immunoprecipitate using APTX antibody (CAB5364) at a dilition of 1:500. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IF |
Application: |
IP |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
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