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APTX Polyclonal Antibody (CAB5364)

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CAB5364
  • Western blot - APTX Polyclonal Antibody (CAB5364)
  • Immunofluorescence - APTX Polyclonal Antibody (CAB5364)
  • Immunoprecipitation - APTX Polyclonal Antibody (CAB5364)
$331.89

Description

Overview

Title:APTX Polyclonal Antibody (CAB5364)
Size:100µL
Code:CAB5364
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
GeneID:54840
SwissProt:Q7Z2E3
Synonyms:APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; aprataxin
Calculated MW:5kDa/13kDa/19-40kDa
Observed MW:40kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:22Rv1, A-549, K-562, BT-474, U-87MG, HT-29, Raji
Cellular Location:Cytoplasm, Nucleus, nucleolus, nucleoplasm

Antibody Images

Western blot - APTX Polyclonal Antibody (CAB5364)

Western blot analysis of extracts of various cell lines, using APTX antibody (CAB5364) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - APTX Polyclonal Antibody (CAB5364)

Immunofluorescence analysis of A549 cells using APTX antibody (CAB5364).

Immunoprecipitation - APTX Polyclonal Antibody (CAB5364)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (CAB5364). Western blot was performed from the immunoprecipitate using APTX antibody (CAB5364) at a dilition of 1:500.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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