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APP Polyclonal Antibody (CAB0206)

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CAB0206
  • Western blot - APP Polyclonal Antibody (CAB0206)
  • Immunohistochemistry - APP Polyclonal Antibody (CAB0206)
  • Immunohistochemistry - APP Polyclonal Antibody (CAB0206)
$331.89

Description

Overview

Title:APP Polyclonal Antibody (CAB0206)
Size:100µL
Code:CAB0206
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant protein of human APP
GeneID:351
SwissProt:P05067
Synonyms:APP; AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; preA4; amyloid beta A4 protein
Calculated MW:34kDa/72-86kDa
Observed MW:110kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:U-87MG, 293T, LO2, HL-60
Cellular Location:Membrane, Single-pass type I membrane protein, clathrin-coated pit

Antibody Images

Western blot - APP Polyclonal Antibody (CAB0206)

Western blot analysis of extracts of various cell lines, using APP antibody (CAB0206) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - APP Polyclonal Antibody (CAB0206)

Immunohistochemistry of paraffin-embedded human stomach using APP antibody (CAB0206) at dilution of 1:100 (40x lens).

Immunohistochemistry - APP Polyclonal Antibody (CAB0206)

Immunohistochemistry of paraffin-embedded human esophagus using APP antibody (CAB0206) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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