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APOBEC3B Polyclonal Antibody (CAB9010)

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SKU:
CAB9010
$331.89

Description

Overview

Title:APOBEC3B Polyclonal Antibody (CAB9010)
Size:100µL
Code:CAB9010
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APOBEC3B (NP_004891.4).
GeneID:9582
SwissProt:Q9UH17
Synonyms:APOBEC3B; A3B; APOBEC1L; ARCD3; ARP4; DJ742C19.2; PHRBNL; bK150C2.2; DNA dC->dU-editing enzyme APOBEC-3B
Calculated MW:29kDa/43kDa/45kDa
Observed MW:46kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:1000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, U-251MG, MCF7
Cellular Location:Nucleus

Antibody Images

Western blot - APOBEC3B Polyclonal Antibody (CAB9010)

Western blot analysis of extracts of various cell lines, using APOBEC3B antibody (CAB9010) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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