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APOBEC3A Polyclonal Antibody (CAB12399)

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SKU:
CAB12399
$331.89

Description

Overview

Title:APOBEC3A Polyclonal Antibody (CAB12399)
Size:100µL
Code:CAB12399
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human APOBEC3A (NP_663745.1).
GeneID:200315
SwissProt:P31941
Synonyms:APOBEC3A; A3A; ARP3; PHRBN; bK150C2.1; DNA dC->dU-editing enzyme APOBEC-3A
Calculated MW:21kDa/23kDa
Observed MW:25kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:500
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HT-29
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - APOBEC3A Polyclonal Antibody (CAB12399)

Western blot analysis of extracts of HT-29 cells, using APOBEC3A antibody (CAB12399) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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