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APITD1 Polyclonal Antibody (CAB8293)

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SKU:
CAB8293
$331.89

Description

Overview

Title:APITD1 Polyclonal Antibody (CAB8293)
Size:100µL
Code:CAB8293
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene was identified in the neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumors, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. The protein is a component of multiple complexes, including the Fanconi anemia (FA) core complex, the APITD1/CENPS complex, and the CENPA-CAD (nucleosome distal) complex. Known functions include an involvement with chromatin associations of the FA core complex, and a role in the stable assembly of the outer kinetochore. Alternative splicing of this gene results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream cortistatin (CORT) gene, as represented in GeneID:100526739. An APITD1-related pseudogene has been identified on chromosome 7.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human APITD1 (NP_954988.1).
GeneID:378708
SwissProt:Q8N2Z9
Synonyms:CENPS; APITD1; CENP-S; FAAP16; MHF1; centromere protein S
Calculated MW:9kDa/15kDa/19kDa
Observed MW:16kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-251MG, SW620, NCI-H460, MCF7, A-549
Cellular Location:Chromosome, Nucleus, centromere, kinetochore

Antibody Images

Western blot - APITD1 Polyclonal Antibody (CAB8293)

Western blot analysis of extracts of various cell lines, using APITD1 antibody (CAB8293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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