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APEX1 Monoclonal Antibody (CAB0476)

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SKU:
CAB0476
$331.89

Description

Overview

Title:APEX1 Monoclonal Antibody (CAB0476)
Size:100µL
Code:CAB0476
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Immunogen Information

Immunogen:A synthetic peptide of human APEX1
GeneID:328
SwissProt:P27695
Synonyms:APEX1; APE; APE1; APEN; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase
Calculated MW:35kDa
Observed MW:36kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:20 - 1:50; IF 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:MCF-7
Cellular Location:Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Nucleus speckle, nucleolus

Antibody Images

Western blot - APEX1 Monoclonal Antibody (CAB0476)

Western blot analysis of extracts of MCF-7 cells, using APEX1 antibody (CAB0476).Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG
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