APBB1 Polyclonal Antibody (CAB1944)

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CAB1944
  • Western blot - APBB1 Polyclonal Antibody (CAB1944)
  • Immunohistochemistry - APBB1 Polyclonal Antibody (CAB1944)
  • Immunofluorescence - APBB1 Polyclonal Antibody (CAB1944)
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Description

Antibody DatasheetAntibody MSDS

APBB1 Polyclonal Antibody (CAB1944)

Overview

Title:APBB1 Polyclonal Antibody (CAB1944)
Size:100µL
Code:CAB1944
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 359-708 of human APBB1 (NP_663722.1).
GeneID:322
SwissProt:O00213
Synonyms:APBB1; FE65; MGC:9072; RIR; amyloid beta precursor protein binding family B member 1
Calculated MW:49kDa/52kDa/53kDa/54kDa/76kDa/77kDa
Observed MW:63kDa

Applications

Reactivity:Human
Tested Applications:IF
Recommended Dilution:IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Immunofluorescence    
Positive Samples:Raji, LOVO, BT-474, A-549, Mouse brain, Mouse heart, Mouse lung, Mouse spinal cord
Cellular Location:Cell membrane, Cell projection, Cytoplasm, Nucleus, Nucleus speckle, growth cone

Antibody Images

Western blot - APBB1 Polyclonal Antibody (CAB1944)

Western blot analysis of extracts of various cell lines, using APBB1 antibody (CAB1944) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - APBB1 Polyclonal Antibody (CAB1944)

Immunohistochemistry of paraffin-embedded human colon carcinoma using APBB1 antibody (CAB1944) at dilution of 1:100 (40x lens).

Immunofluorescence - APBB1 Polyclonal Antibody (CAB1944)

Immunofluorescence analysis of U2OS cells using APBB1 antibody (CAB1944). Blue: DAPI for nuclear staining.

APBB1 Polyclonal Antibody (CAB1944) Protein Information

UniProt Protein Function:Fe65: adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on Y142 (H2AXpY142) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as TIP60, probably explains its trancription activation activity.
UniProt Protein Details:

Protein type:Apoptosis; Adaptor/scaffold; Transcription regulation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: growth cone; lamellipodium; cytoplasm; plasma membrane; nuclear speck; synapse; nucleus

Molecular Function:protein binding; histone binding; beta-amyloid binding; chromatin binding; transcription factor binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; positive regulation of DNA repair; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; neuron migration; signal transduction; negative regulation of thymidylate synthase biosynthetic process; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; axonogenesis; double-strand break repair; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell cycle arrest; response to DNA damage stimulus

NCBI Summary:The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:O00213
NCBI GenInfo Identifier:12229629
NCBI Gene ID:322
NCBI Accession:O00213.2
UniProt Secondary Accession:O00213,Q7Z324, Q96A93, A1E379, A6NH82, A6NL69, B7Z1J5 B7Z1J6, B7Z2Y0, D3DQT2, V9GYK0, V9GYT4,
UniProt Related Accession:O00213
Molecular Weight:52,656 Da
NCBI Full Name:Amyloid beta A4 protein-binding family B member 1
NCBI Synonym Full Names:amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
NCBI Official Symbol:APBB1  
NCBI Official Synonym Symbols:RIR; FE65; MGC:9072  
NCBI Protein Information:amyloid beta A4 precursor protein-binding family B member 1; stat-like protein; adaptor protein FE65a2
UniProt Protein Name:Amyloid beta A4 precursor protein-binding family B member 1
UniProt Synonym Protein Names:Protein Fe65
Protein Family:Amyloid beta A4 precursor protein-binding family
UniProt Gene Name:APBB1  
UniProt Entry Name:APBB1_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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