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APBB1 Polyclonal Antibody (CAB1944)

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CAB1944
  • Western blot - APBB1 Polyclonal Antibody (CAB1944)
  • Immunohistochemistry - APBB1 Polyclonal Antibody (CAB1944)
  • Immunofluorescence - APBB1 Polyclonal Antibody (CAB1944)
$331.89

Description

Overview

Title:APBB1 Polyclonal Antibody (CAB1944)
Size:100µL
Code:CAB1944
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 359-708 of human APBB1 (NP_663722.1).
GeneID:322
SwissProt:O00213
Synonyms:APBB1; FE65; MGC:9072; RIR; amyloid beta precursor protein binding family B member 1
Calculated MW:49kDa/52kDa/53kDa/54kDa/76kDa/77kDa
Observed MW:63kDa

Applications

Reactivity:Human
Tested Applications:IF
Recommended Dilution:IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Immunofluorescence    
Positive Samples:Raji, LOVO, BT-474, A-549, Mouse brain, Mouse heart, Mouse lung, Mouse spinal cord
Cellular Location:Cell membrane, Cell projection, Cytoplasm, Nucleus, Nucleus speckle, growth cone

Antibody Images

Western blot - APBB1 Polyclonal Antibody (CAB1944)

Western blot analysis of extracts of various cell lines, using APBB1 antibody (CAB1944) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - APBB1 Polyclonal Antibody (CAB1944)

Immunohistochemistry of paraffin-embedded human colon carcinoma using APBB1 antibody (CAB1944) at dilution of 1:100 (40x lens).

Immunofluorescence - APBB1 Polyclonal Antibody (CAB1944)

Immunofluorescence analysis of U2OS cells using APBB1 antibody (CAB1944). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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