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AP1M1 Polyclonal Antibody (CAB10129)

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SKU:
CAB10129
$331.89

Description

Overview

Title:AP1M1 Polyclonal Antibody (CAB10129)
Size:100µL
Code:CAB10129
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human AP1M1 (NP_115882.1).
GeneID:8907
SwissProt:Q9BXS5
Synonyms:AP1M1; AP47; CLAPM2; CLTNM; MU-1A; AP-1 complex subunit mu-1
Calculated MW:48kDa/49kDa
Observed MW:49kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:4000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, Jurkat, HepG2, HL-60, HT-1080, Mouse brain, Mouse kidney, Mouse liver, Rat testis
Cellular Location:Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane

Antibody Images

Western blot - AP1M1 Polyclonal Antibody (CAB10129)

Western blot analysis of extracts of various cell lines, using AP1M1 antibody (CAB10129) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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