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Annexin A11 Polyclonal Antibody (CAB7423)

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CAB7423
  • Western blot - ANXA11 Polyclonal Antibody (CAB7423)
  • Immunofluorescence - ANXA11 Polyclonal Antibody (CAB7423)
  • Immunoprecipitation - ANXA11 Polyclonal Antibody (CAB7423)
€299.00

Description

Overview

Title:ANXA11 Polyclonal Antibody (CAB7423)
Size:100µL
Code:CAB7423
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11 (NP_001148.1).
GeneID:311
SwissProt:P50995
Synonyms:ANXA11; ANX11; CAP50; annexin A11
Calculated MW:51kDa/54kDa
Observed MW:56kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:A-431, HT-29, Jurkat, SKOV3, A-549, Mouse brain, Mouse skeletal muscle, Rat brain
Cellular Location:Cytoplasm, Melanosome, Nucleus, Nucleus envelope, cytoskeleton, nucleoplasm, spindle

Antibody Images

Western blot - ANXA11 Polyclonal Antibody (CAB7423)

Western blot analysis of extracts of various cell lines, using ANXA11 antibody (CAB7423) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - ANXA11 Polyclonal Antibody (CAB7423)

Immunofluorescence analysis of A549 cells using ANXA11 antibody (CAB7423). Blue: DAPI for nuclear staining.

Immunoprecipitation - ANXA11 Polyclonal Antibody (CAB7423)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody (CAB7423). Western blot was performed from the immunoprecipitate using ANXA11 antibody (CAB7423) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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