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ANLN Polyclonal Antibody (CAB6524)

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CAB6524
  • Western blot - ANLN Polyclonal Antibody (CAB6524)
  • Immunofluorescence - ANLN Polyclonal Antibody (CAB6524)
$331.89

Description

Overview

Title:ANLN Polyclonal Antibody (CAB6524)
Size:100µL
Code:CAB6524
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 845-1124 of human ANLN (NP_061155.2).
GeneID:54443
SwissProt:Q9NQW6
Synonyms:ANLN; FSGS8; Scraps; scra; anillin
Calculated MW:120kDa/124kDa
Observed MW:120kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, MCF-7
Cellular Location:Cytoplasm, Nucleus, cell cortex, cytoskeleton

Antibody Images

Western blot - ANLN Polyclonal Antibody (CAB6524)

Western blot analysis of extracts of various cell lines, using ANLN antibody (CAB6524) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunofluorescence - ANLN Polyclonal Antibody (CAB6524)

Immunofluorescence analysis of A549 cells using ANLN antibody (CAB6524).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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