ANAPC5 Polyclonal Antibody (CAB7109)

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CAB7109
  • Western blot - ANAPC5 Polyclonal Antibody (CAB7109)
  • Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)
  • Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)
  • Immunoprecipitation - ANAPC5 Polyclonal Antibody (CAB7109)
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Description

Antibody DatasheetAntibody MSDS

ANAPC5 Polyclonal Antibody (CAB7109)

Overview

Title:ANAPC5 Polyclonal Antibody (CAB7109)
Size:100µL
Code:CAB7109
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
GeneID:51433
SwissProt:Q9UJX4
Synonyms:ANAPC5; APC5; anaphase-promoting complex subunit 5
Calculated MW:25kDa/72kDa/85kDa
Observed MW:85kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:SW620, MCF-7, HL-60, 22Rv1, A-549, Mouse spleen
Cellular Location:N/A

Antibody Images

Western blot - ANAPC5 Polyclonal Antibody (CAB7109)

Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (CAB7109) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)

Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens).

Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)

Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens).

Immunoprecipitation - ANAPC5 Polyclonal Antibody (CAB7109)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (CAB7109). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (CAB7109) at a dilition of 1:1000.

ANAPC5 Polyclonal Antibody (CAB7109) Protein Information

UniProt Protein Function:APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol

Molecular Function:protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

NCBI Summary:This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]
UniProt Code:Q9UJX4
NCBI GenInfo Identifier:37537861
NCBI Gene ID:51433
NCBI Accession:Q9UJX4.2
UniProt Secondary Accession:Q9UJX4,Q8N4H7, Q9BQD4, E9PFB2,
UniProt Related Accession:Q9UJX4
Molecular Weight:72,526 Da
NCBI Full Name:Anaphase-promoting complex subunit 5
NCBI Synonym Full Names:anaphase promoting complex subunit 5
NCBI Official Symbol:ANAPC5  
NCBI Official Synonym Symbols:APC5  
NCBI Protein Information:anaphase-promoting complex subunit 5; cyclosome subunit 5
UniProt Protein Name:Anaphase-promoting complex subunit 5
UniProt Synonym Protein Names:Cyclosome subunit 5
UniProt Gene Name:ANAPC5  
UniProt Entry Name:APC5_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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