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ANAPC5 Polyclonal Antibody (CAB7109)

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CAB7109
  • Western blot - ANAPC5 Polyclonal Antibody (CAB7109)
  • Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)
  • Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)
  • Immunoprecipitation - ANAPC5 Polyclonal Antibody (CAB7109)
$331.89

Description

Overview

Title:ANAPC5 Polyclonal Antibody (CAB7109)
Size:100µL
Code:CAB7109
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
GeneID:51433
SwissProt:Q9UJX4
Synonyms:ANAPC5; APC5; anaphase-promoting complex subunit 5
Calculated MW:25kDa/72kDa/85kDa
Observed MW:85kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:SW620, MCF-7, HL-60, 22Rv1, A-549, Mouse spleen
Cellular Location:N/A

Antibody Images

Western blot - ANAPC5 Polyclonal Antibody (CAB7109)

Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (CAB7109) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)

Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens).

Immunohistochemistry - ANAPC5 Polyclonal Antibody (CAB7109)

Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens).

Immunoprecipitation - ANAPC5 Polyclonal Antibody (CAB7109)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (CAB7109). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (CAB7109) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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