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ANAPC5 Polyclonal Antibody (CAB4736)

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SKU:
CAB4736
$331.89

Description

Overview

Title:ANAPC5 Polyclonal Antibody (CAB4736)
Size:100µL
Code:CAB4736
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Immunogen Information

Immunogen:A synthetic Peptide of human ANAPC5
GeneID:51433
SwissProt:Q9UJX4
Synonyms:ANAPC5; APC5; anaphase-promoting complex subunit 5
Calculated MW:25kDa/72kDa/85kDa
Observed MW:85kDa

Applications

Reactivity:Human
Tested Applications:WB; FC
Recommended Dilution:WB 1:500 - 1:1000; FC 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Flow Cytometry    
Positive Samples:A-549
Cellular Location:N/A

Antibody Images

Western blot - ANAPC5 Polyclonal Antibody (CAB4736)

Western blot analysis of extracts of A-549 cells, using ANAPC5 antibody (CAB4736).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
FC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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