ANAPC5 Polyclonal Antibody (CAB4736)
- SKU:
- CAB4736
Description
| Title: | ANAPC5 Polyclonal Antibody (CAB4736) | |
| Size: | 100µL | |
| Code: | CAB4736 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
Immunogen Information
| Immunogen: | A synthetic Peptide of human ANAPC5 |
| GeneID: | 51433 |
| SwissProt: | Q9UJX4 |
| Synonyms: | ANAPC5; APC5; anaphase-promoting complex subunit 5 |
| Calculated MW: | 25kDa/72kDa/85kDa |
| Observed MW: | 85kDa |
Applications
| Reactivity: | Human |
| Tested Applications: | WB; FC |
| Recommended Dilution: | WB 1:500 - 1:1000; FC 1:20 - 1:50 |
| Storage Buffer: | Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3. |
| Application Key: | Western blotting Flow Cytometry |
| Positive Samples: | A-549 |
| Cellular Location: | N/A |
Antibody Images
| Western blot analysis of extracts of A-549 cells, using ANAPC5 antibody (CAB4736).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
FC |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |
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