ANAPC4 Polyclonal Antibody (CAB8962)

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CAB8962
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Description

Antibody DatasheetAntibody MSDS

ANAPC4 Polyclonal Antibody (CAB8962)

Overview

Title:ANAPC4 Polyclonal Antibody (CAB8962)
Size:100µL
Code:CAB8962
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:A synthetic Peptide of human ANAPC4
GeneID:29945
SwissProt:Q9UJX5
Synonyms:ANAPC4; APC4; anaphase-promoting complex subunit 4
Calculated MW:62kDa/92kDa
Observed MW:Refer to figures

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:1000
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    
Positive Samples:N/A
Cellular Location:N/A

ANAPC4 Polyclonal Antibody (CAB8962) Protein Information

UniProt Protein Function:APC4: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC4 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol

Molecular Function:protein binding; ubiquitin-protein ligase activity; protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

NCBI Summary:A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Code:Q9UJX5
NCBI GenInfo Identifier:205371737
NCBI Gene ID:29945
NCBI Accession:Q9UJX5.2
UniProt Related Accession:Q9UJX5
Molecular Weight:
NCBI Full Name:Anaphase-promoting complex subunit 4
NCBI Synonym Full Names:anaphase promoting complex subunit 4
NCBI Official Symbol:ANAPC4  
NCBI Official Synonym Symbols:APC4  
NCBI Protein Information:anaphase-promoting complex subunit 4
UniProt Protein Name:Anaphase-promoting complex subunit 4
UniProt Synonym Protein Names:Cyclosome subunit 4
Protein Family:Acetophenone carboxylase
UniProt Gene Name:ANAPC4  
UniProt Entry Name:APC4_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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