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ANAPC13 Polyclonal Antibody (CAB9967)

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SKU:
CAB9967
$331.89

Description

Overview

Title:ANAPC13 Polyclonal Antibody (CAB9967)
Size:100µL
Code:CAB9967
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-74 of human ANAPC13 (NP_056206.1).
GeneID:25847
SwissProt:Q9BS18
Synonyms:ANAPC13; APC13; SWM1; anaphase-promoting complex subunit 13
Calculated MW:8kDa
Observed MW:15kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:293T, MCF-7, Mouse heart
Cellular Location:Nucleus

Antibody Images

Western blot - ANAPC13 Polyclonal Antibody (CAB9967)

Western blot analysis of extracts of various cell lines, using ANAPC13 antibody (CAB9967) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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