ANAPC13 Polyclonal Antibody (CAB9967)

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CAB9967
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Description

Antibody DatasheetAntibody MSDS

ANAPC13 Polyclonal Antibody (CAB9967)

Overview

Title:ANAPC13 Polyclonal Antibody (CAB9967)
Size:100µL
Code:CAB9967
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-74 of human ANAPC13 (NP_056206.1).
GeneID:25847
SwissProt:Q9BS18
Synonyms:ANAPC13; APC13; SWM1; anaphase-promoting complex subunit 13
Calculated MW:8kDa
Observed MW:15kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:293T, MCF-7, Mouse heart
Cellular Location:Nucleus

Antibody Images

Western blot - ANAPC13 Polyclonal Antibody (CAB9967)

Western blot analysis of extracts of various cell lines, using ANAPC13 antibody (CAB9967) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

ANAPC13 Polyclonal Antibody (CAB9967) Protein Information

UniProt Protein Function:APC13: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC13 family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.2

Cellular Component: anaphase-promoting complex

Biological Process: mitosis; cell division

NCBI Summary:This gene encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined. [provided by RefSeq, Nov 2008]
UniProt Code:Q9BS18
NCBI GenInfo Identifier:74732956
NCBI Gene ID:25847
NCBI Accession:Q9BS18.1
UniProt Secondary Accession:Q9BS18,Q9Y3V0,
UniProt Related Accession:Q9BS18
Molecular Weight:8,521 Da
NCBI Full Name:Anaphase-promoting complex subunit 13
NCBI Synonym Full Names:anaphase promoting complex subunit 13
NCBI Official Symbol:ANAPC13  
NCBI Official Synonym Symbols:SWM1; APC13  
NCBI Protein Information:anaphase-promoting complex subunit 13; cyclosome subunit 13
UniProt Protein Name:Anaphase-promoting complex subunit 13
UniProt Synonym Protein Names:Cyclosome subunit 13
UniProt Gene Name:ANAPC13  
UniProt Entry Name:APC13_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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