ANAPC1 Polyclonal Antibody (CAB3484)

(No reviews yet) Write a Review
SKU:
CAB3484
€299.00
Frequently bought together:

Description

Antibody DatasheetAntibody MSDS

ANAPC1 Polyclonal Antibody (CAB3484)

Overview

Title:ANAPC1 Polyclonal Antibody (CAB3484)
Size:100µL
Code:CAB3484
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC1 (NP_073153.1).
GeneID:64682
SwissProt:Q9H1A4
Synonyms:ANAPC1; APC1; MCPR; TSG24; anaphase-promoting complex subunit 1
Calculated MW:216kDa
Observed MW:217kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Jurkat, LO2
Cellular Location:N/A

Antibody Images

Western blot - ANAPC1 Polyclonal Antibody (CAB3484)

Western blot analysis of extracts of various cell lines, using ANAPC1 antibody (CAB3484) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

ANAPC1 Polyclonal Antibody (CAB3484) Protein Information

UniProt Protein Function:APC1: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC1 family.
UniProt Protein Details:

Protein type:Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q12.1

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

NCBI Summary:This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]
UniProt Code:Q9H1A4
NCBI GenInfo Identifier:37537845
NCBI Gene ID:64682
NCBI Accession:Q9H1A4.1
UniProt Secondary Accession:Q9H1A4,Q2M3H8, Q9BSE6, Q9H8D0,
UniProt Related Accession:Q9H1A4
Molecular Weight:1944
NCBI Full Name:Anaphase-promoting complex subunit 1
NCBI Synonym Full Names:anaphase promoting complex subunit 1
NCBI Official Symbol:ANAPC1  
NCBI Official Synonym Symbols:APC1; MCPR; TSG24  
NCBI Protein Information:anaphase-promoting complex subunit 1; cyclosome subunit 1; mitotic checkpoint regulator; testis-specific gene 24 protein; anaphase-promoting complex 1 (meiotic checkpoint regulator)
UniProt Protein Name:Anaphase-promoting complex subunit 1
UniProt Synonym Protein Names:Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein
UniProt Gene Name:ANAPC1  
UniProt Entry Name:APC1_HUMAN
View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
View AllClose