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ANAPC1 Polyclonal Antibody (CAB3484)

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SKU:
CAB3484
$331.89

Description

Overview

Title:ANAPC1 Polyclonal Antibody (CAB3484)
Size:100µL
Code:CAB3484
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC1 (NP_073153.1).
GeneID:64682
SwissProt:Q9H1A4
Synonyms:ANAPC1; APC1; MCPR; TSG24; anaphase-promoting complex subunit 1
Calculated MW:216kDa
Observed MW:217kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Jurkat, LO2
Cellular Location:N/A

Antibody Images

Western blot - ANAPC1 Polyclonal Antibody (CAB3484)

Western blot analysis of extracts of various cell lines, using ANAPC1 antibody (CAB3484) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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