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alpha Tubulin Polyclonal Antibody (CABC025)

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SKU:
CABC025
$398.49

Description

Overview

Title:alpha Tubulin Polyclonal Antibody (CABC025)
Size:100uL
Code:CABC025
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human alpha Tubulin (NP_006073.2).
GeneID:10376
SwissProt:P68363
Synonyms:TUBA1B; K-ALPHA-1; tubulin alpha 1b
Calculated MW:37kDa/50kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB;
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HT-29, Mouse eye
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - alpha Tubulin Polyclonal Antibody (CABC025)

Western blot analysis of extracts of various cell lines, using alpha Tubulin antibody (CABC025) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
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Additional Information

ANTIBODY TYPE:
Polyclonal
APPLICATION:
WB
REACTIVITY:
Human
REACTIVITY:
Mouse
HOST SPECIES:
Rabbit
ISOTYPE:
IgG
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