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alpha Tubulin Monoclonal Antibody (CABC012)

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CABC012
  • Western blot - alpha Tubulin Monoclonal Antibody (CABC012)
  • Immunofluorescence - alpha Tubulin Monoclonal Antibody (CABC012)
$331.89

Description

Overview

Title:alpha Tubulin Monoclonal Antibody (CABC012)
Size:100uL
Code:CABC012
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant protein of human TUBA1B
GeneID:7277
SwissProt:P68366
Synonyms:TUBA4A; ALS22; H2-ALPHA; TUBA1; tubulin alpha 4a
Calculated MW:48kDa/49kDa
Observed MW:50kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP;
Recommended Dilution:WB 1:5000 - 1:10000; IHC 1:200 - 1:500; IF 1:100 - 1:200; IP 1:100 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:U-87MG, 293T, HeLa, Jurkat, Mouse spleen, Mouse thymus, Mouse brain, Rat brain, Rat kidney
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - alpha Tubulin Monoclonal Antibody (CABC012)

Western blot analysis of extracts of various cell lines, using alpha Tubulin antibody (CABC012) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - alpha Tubulin Monoclonal Antibody (CABC012)

Immunofluorescence analysis of HeLa cells using TUBA4A antibody (CABC012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Mouse
ISOTYPE:
IgG
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