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Akt1 Polyclonal Antibody (CAB5523)

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CAB5523
  • Western blot - Akt1 Polyclonal Antibody (CAB5523)
  • Immunofluorescence - Akt1 Polyclonal Antibody (CAB5523)
$331.89

Description

Overview

Title:Akt1 Polyclonal Antibody (CAB5523)
Size:100µL
Code:CAB5523
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen Information

Immunogen:Recombinant protein of human AKT1
GeneID:207
SwissProt:P31749
Synonyms:AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT serine/threonine kinase 1
Calculated MW:48kDa/55kDa
Observed MW:65kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:SKOV3, A-431, Mouse brain, Rat thymus, Rat liver
Cellular Location:Cell membrane, Cytoplasm, Nucleus

Antibody Images

Western blot - Akt1 Polyclonal Antibody (CAB5523)

Western blot analysis of extracts of various cell lines, using Akt1 antibody (CAB5523) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - Akt1 Polyclonal Antibody (CAB5523)

Immunofluorescence analysis of A431 cells using AKT1 antibody (CAB5523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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