The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

AIMP1 Polyclonal Antibody (CAB5301)

(No reviews yet) Write a Review
SKU:
CAB5301
  • Western blot - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)
  • Immunofluorescence - AIMP1 Polyclonal Antibody (CAB5301)
$331.89

Description

Overview

Title:AIMP1 Polyclonal Antibody (CAB5301)
Size:100µL
Code:CAB5301
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human AIMP1 (NP_001135888.1).
GeneID:9255
SwissProt:Q12904
Synonyms:AIMP1; EMAP2; EMAPII; HLD3; SCYE1; p43; aminoacyl tRNA synthase complex-interacting multifunctional protein 1
Calculated MW:34kDa/37kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HL-60, 22RV1, HepG2, HT-29, MCF7, A-549
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, cytosol, secretory vesicle

Antibody Images

Western blot - AIMP1 Polyclonal Antibody (CAB5301)

Western blot analysis of extracts of various cell lines, using AIMP1 Antibody (CAB5301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded rat spleen using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded rat kidney using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded rat heart using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded human liver cancer using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded human gastric cancer using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded mouse kidney using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunohistochemistry - AIMP1 Polyclonal Antibody (CAB5301)

Immunohistochemistry of paraffin-embedded mouse heart using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).

Immunofluorescence - AIMP1 Polyclonal Antibody (CAB5301)

Immunofluorescence analysis of HeLa cells using AIMP1 antibody (CAB5301). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose