AIM2 Polyclonal Antibody (CAB3356)

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CAB3356
€299.00
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Description

Antibody DatasheetAntibody MSDS

AIM2 Polyclonal Antibody (CAB3356)

Overview

Title:AIM2 Polyclonal Antibody (CAB3356)
Size:100µL
Code:CAB3356
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AIM2 (NP_004824.1).
GeneID:9447
SwissProt:O14862
Synonyms:AIM2; PYHIN4; absent in melanoma 2
Calculated MW:38kDa
Observed MW:47kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, HepG2
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - AIM2 Polyclonal Antibody (CAB3356)

Western blot analysis of extracts of various cell lines, using AIM2 antibody (CAB3356) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

AIM2 Polyclonal Antibody (CAB3356) Protein Information

UniProt Protein Function:AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.
UniProt Protein Details:

Protein type:Tumor suppressor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function:identical protein binding; protein binding; double-stranded DNA binding

Biological Process: positive regulation of interleukin-1 beta production; positive regulation of protein oligomerization; tumor necrosis factor-mediated signaling pathway; apoptosis; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta secretion; immune response; positive regulation of interleukin-1 beta secretion; inflammatory response; positive regulation of defense response to virus by host; activation of innate immune response; activation of NF-kappaB transcription factor

NCBI Summary:AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]
UniProt Code:O14862
NCBI GenInfo Identifier:5902751
NCBI Gene ID:9447
NCBI Accession:O14862.1
UniProt Secondary Accession:O14862,Q5T3V9, Q96FG9, A8K7M7,
UniProt Related Accession:O14862
Molecular Weight:343
NCBI Full Name:Interferon-inducible protein AIM2
NCBI Synonym Full Names:absent in melanoma 2
NCBI Official Symbol:AIM2  
NCBI Official Synonym Symbols:PYHIN4  
NCBI Protein Information:interferon-inducible protein AIM2
UniProt Protein Name:Interferon-inducible protein AIM2
UniProt Synonym Protein Names:Absent in melanoma 2
Protein Family:Protein
UniProt Gene Name:AIM2  
UniProt Entry Name:AIM2_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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