Description
Title: | AIFM1 Polyclonal Antibody (CAB0811) | |
Size: | 100µL | |
Code: | CAB0811 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
Immunogen Information
Immunogen: | A synthetic peptide of human AIFM1 |
GeneID: | 9131 |
SwissProt: | O95831 |
Synonyms: | AIFM1; AIF; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; apoptosis-inducing factor 1, mitochondrial |
Calculated MW: | 26kDa/28kDa/35kDa/66kDa |
Observed MW: | 60kDa |
Applications
Reactivity: | Human |
Tested Applications: | WB; IHC; IF; FC |
Recommended Dilution: | WB 1:500 - 1:1000; IHC 1:50 - 1:200; IF 1:20 - 1:50; FC 1:20 - 1:50 |
Storage Buffer: | Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3. |
Application Key: | Western blotting Immunohistochemistry Immunofluorescence Flow Cytometry |
Positive Samples: | HepG2 |
Cellular Location: | Cytoplasm, Mitochondrion inner membrane, Mitochondrion intermembrane space, Nucleus, perinuclear region |
Antibody Images
Western blot analysis of extracts of HepG2 cells, using AIFM1 antibody (CAB0811).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Application: |
FC |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |