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AF/LE Purified Mouse H-2 Monoclonal Antibody [M1/](AGEL2108)

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Title:AF/LE Purified Mouse H-2 Monoclonal Antibody
Host Species:Rat
Size:1 mg
Volume Per Test:1mg/mL


The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Antibody Information:

Isotype:IgG2a, ?
Synonyms:Mouse major histocompatibility complex (MHC) H-2, MHC I
Conjugation:Azide Free/Low endotoxin


Tested Applications:Block, FC, IF, IP
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ? 2.0 µg per 10e6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (<0.002EU/?g) as Determined by LAL gel clotting assay. Purity is >95% as determined by SDS-PAGE
Storage:Store at 2~8?. Do not freeze.
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