Description
Title: | AF/LE Purified Human CD279/PD-1 Monoclonal Antibody |
Host Species: | Mouse |
Size: | 1 mg |
Volume Per Test: | 1mg/mL |
Background:
Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.Isotype: | IgG2a, ? |
GeneID: | 5133 |
SwissProt: | Q15116 |
Synonyms: | PD-1, Programmed Death-1 |
Clone: | J116 |
Conjugation: | Azide Free/Low endotoxin |
Reactivity: | Human |
Tested Applications: | Block, Neut, FC,IHC,IP |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ? 2.0 µg per 10e6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Storage Buffer: | 0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (<0.002EU/?g) as Determined by LAL gel clotting assay. Purity is >95% as determined by SDS-PAGE |
Storage: | Store at 2~8?. Do not freeze. |