|Title:||AF/LE Purified Human CD11a Monoclonal Antibody|
|Volume Per Test:||1mg/mL|
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1alpha chain and integrin alphaL subunit. CD11a non-covalently associates with integrin beta2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
|Conjugation:||Azide Free/Low endotoxin|
|Tested Applications:||FA, FC|
|Recommended Dilution:||Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ? 2.0 µg per 10e6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.|
|Storage Buffer:||0.2 µm filtered in PBS, pH7.2. Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg (<0.002EU/?g) as Determined by LAL gel clotting assay. Purity is >95% as determined by SDS-PAGE |
|Storage:||Store at 2~8?. Do not freeze. |