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ACTL6B Polyclonal Antibody (CAB7108)

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CAB7108
  • Western blot - ACTL6B Polyclonal Antibody (CAB7108)
  • Immunohistochemistry - ACTL6B Polyclonal Antibody (CAB7108)
  • Immunohistochemistry - ACTL6B Polyclonal Antibody (CAB7108)
  • Immunohistochemistry - ACTL6B Polyclonal Antibody (CAB7108)
  • Immunofluorescence - ACTL6B Polyclonal Antibody (CAB7108)
$331.89

Description

Overview

Title:ACTL6B Polyclonal Antibody (CAB7108)
Size:100µL
Code:CAB7108
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6B (NP_057272.1).
GeneID:51412
SwissProt:O94805
Synonyms:ACTL6B; ACTL6; BAF53B; arpNalpha; actin like 6B
Calculated MW:46kDa
Observed MW:47kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HL-60, 22Rv1, BT-474, HeLa, HepG2, Mouse brain, Mouse skeletal muscle
Cellular Location:Nucleus

Antibody Images

Western blot - ACTL6B Polyclonal Antibody (CAB7108)

Western blot analysis of extracts of various cell lines, using ACTL6B antibody (CAB7108) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.

Immunohistochemistry - ACTL6B Polyclonal Antibody (CAB7108)

Immunohistochemistry of paraffin-embedded human kidney using ACTL6B antibody (CAB7108) at dilution of 1:100 (40x lens).

Immunohistochemistry - ACTL6B Polyclonal Antibody (CAB7108)

Immunohistochemistry of paraffin-embedded human kidney cancer using ACTL6B antibody (CAB7108) at dilution of 1:100 (40x lens).

Immunohistochemistry - ACTL6B Polyclonal Antibody (CAB7108)

Immunohistochemistry of paraffin-embedded mouse jejunum using ACTL6B antibody (CAB7108) at dilution of 1:100 (40x lens).

Immunofluorescence - ACTL6B Polyclonal Antibody (CAB7108)

Immunofluorescence analysis of A-549 cells using ACTL6B antibody (CAB7108).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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