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ACTL6A Polyclonal Antibody (CAB2220)

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SKU:
CAB2220
$331.89

Description

Overview

Title:ACTL6A Polyclonal Antibody (CAB2220)
Size:100µL
Code:CAB2220
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Immunogen Information

Immunogen:A synthetic peptide of human ACTL6A
GeneID:86
SwissProt:O96019
Synonyms:ACTL6A; ACTL6; ARPN-BETA; Arp4; BAF53A; INO80K; actin like 6A
Calculated MW:43kDa/47kDa
Observed MW:50kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, K-562
Cellular Location:Nucleus

Antibody Images

Western blot - ACTL6A Polyclonal Antibody (CAB2220)

Western blot analysis of extracts of various cell lines, using ACTL6A antibody (CAB2220).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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