ACTL6A Polyclonal Antibody (CAB2220)

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CAB2220
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Description

Antibody DatasheetAntibody MSDS

ACTL6A Polyclonal Antibody (CAB2220)

Overview

Title:ACTL6A Polyclonal Antibody (CAB2220)
Size:100µL
Code:CAB2220
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Immunogen Information

Immunogen:A synthetic peptide of human ACTL6A
GeneID:86
SwissProt:O96019
Synonyms:ACTL6A; ACTL6; ARPN-BETA; Arp4; BAF53A; INO80K; actin like 6A
Calculated MW:43kDa/47kDa
Observed MW:50kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, K-562
Cellular Location:Nucleus

Antibody Images

Western blot - ACTL6A Polyclonal Antibody (CAB2220)

Western blot analysis of extracts of various cell lines, using ACTL6A antibody (CAB2220).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

ACTL6A Polyclonal Antibody (CAB2220) Protein Information

UniProt Protein Function:BAF53A: an actin-related protein involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix. A component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of specific genes. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: NuA4 histone acetyltransferase complex; nuclear chromatin; nucleoplasm; nucleus; plasma membrane; protein complex; SWI/SNF complex

Molecular Function:chromatin binding; nucleosomal DNA binding; protein binding

Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; signal transduction

NCBI Summary:This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:O96019
NCBI GenInfo Identifier:23396463
NCBI Gene ID:86
NCBI Accession:O96019.1
UniProt Secondary Accession:O96019,Q8TAE5, Q9BVS8, Q9H0W6, B3KMN1, D3DNR9,
UniProt Related Accession:O96019
Molecular Weight:43,236 Da
NCBI Full Name:Actin-like protein 6A
NCBI Synonym Full Names:actin like 6A
NCBI Official Symbol:ACTL6A  
NCBI Official Synonym Symbols:Arp4; ACTL6; BAF53A; INO80K; ARPN-BETA  
NCBI Protein Information:actin-like protein 6A
UniProt Protein Name:Actin-like protein 6A
UniProt Synonym Protein Names:53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K
Protein Family:Actin-like protein
UniProt Gene Name:ACTL6A  
UniProt Entry Name:ACL6A_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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