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Active Caspase-3 Monoclonal Antibody (CAB11021)

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CAB11021
  • Western blot - Active Caspase-3 Monoclonal Antibody (CAB11021)
  • Immunohistochemistry - Active Caspase-3 Monoclonal Antibody (CAB11021)
  • Immunohistochemistry - Active Caspase-3 Monoclonal Antibody (CAB11021)
  • Immunohistochemistry - Active Caspase-3 Monoclonal Antibody (CAB11021)
  • Immunofluorescence - Active Caspase-3 Monoclonal Antibody (CAB11021)
  • Immunofluorescence - Active Caspase-3 Monoclonal Antibody (CAB11021)
$560.55

Description

Overview

Title:Active Caspase-3 Monoclonal Antibody (CAB11021)
Size:100µL
Code:CAB11021
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Immunogen Information

Immunogen:Recombinant protein of human CASP3
GeneID:836
SwissProt:P42574
Synonyms:CASP3; CPP32; CPP32B; SCA-1; caspase-3
Calculated MW:31kDa
Observed MW:16kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Jurkat
Cellular Location:Cytoplasm

Antibody Images

Western blot - Active Caspase-3 Monoclonal Antibody (CAB11021)

Western blot analysis of extracts of Jurkat cells, using CASP3 antibody (CAB11021).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - Active Caspase-3 Monoclonal Antibody (CAB11021)

Immunohistochemistry of paraffin-embedded human spleen using CASP3 antibody (CAB11021) at dilution of 1:100 (40x lens).

Immunohistochemistry - Active Caspase-3 Monoclonal Antibody (CAB11021)

Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody (CAB11021) at dilution of 1:100 (40x lens).

Immunohistochemistry - Active Caspase-3 Monoclonal Antibody (CAB11021)

Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP3 antibody (CAB11021) at dilution of 1:100 (40x lens).

Immunofluorescence - Active Caspase-3 Monoclonal Antibody (CAB11021)

Immunofluorescence analysis of PC-3M cells using CASP3 antibody (CAB11021).

Immunofluorescence - Active Caspase-3 Monoclonal Antibody (CAB11021)

Immunofluorescence analysis of HeLa cells using CASP3 antibody (CAB11021).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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