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Activated NOTCH1 Monoclonal Antibody (CAB2349)

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SKU:
CAB2349
$331.89

Description

Overview

Title:Activated NOTCH1 Monoclonal Antibody (CAB2349)
Size:50µL
Code:CAB2349
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.

Immunogen Information

Immunogen:A synthetic peptide of human Activated NOTCH1
GeneID:4851
SwissProt:P46531
Synonyms:NOTCH1; AOS5; AOVD1; TAN1; hN1; notch 1
Calculated MW:272kDa
Observed MW:126kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF; FC
Recommended Dilution:WB 1:500 - 1:1000; IF 1:50 - 1:200; FC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Flow Cytometry    
Positive Samples:Molt-4
Cellular Location:Cell membrane, Nucleus, Single-pass type I membrane protein

Antibody Images

Western blot - Activated NOTCH1 Monoclonal Antibody (CAB2349)

Western blot analysis of extracts of Molt-4 cells, using Activated NOTCH1 antibody (CAB2349).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IF
Application:
FC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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