ACTC1 Polyclonal Antibody (CAB6507)

(No reviews yet) Write a Review
SKU:
CAB6507
€299.00
Frequently bought together:

Description

Antibody DatasheetAntibody MSDS

ACTC1 Polyclonal Antibody (CAB6507)

Overview

Title:ACTC1 Polyclonal Antibody (CAB6507)
Size:100µL
Code:CAB6507
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Immunogen Information

Immunogen:A synthetic peptide of human ACTC1
GeneID:70
SwissProt:P68032
Synonyms:ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4; actin, alpha cardiac muscle 1
Calculated MW:42kDa
Observed MW:42kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:10 - 1:50; IF 1:10 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Mouse skeletal muscle
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - ACTC1 Polyclonal Antibody (CAB6507)

Western blot analysis of extracts of mouse skeletal muscle, using ACTC1 antibody (CAB6507).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

ACTC1 Polyclonal Antibody (CAB6507) Protein Information

UniProt Protein Function:ACTC1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.
UniProt Protein Details:

Protein type:Cytoskeletal; Contractile; Cell development/differentiation; Motility/polarity/chemotaxis; Myosin-binding

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: actin filament; cytoplasm; cytosol; extracellular space; filopodium; focal adhesion; I band; lamellipodium; membrane; sarcomere

Molecular Function:ATP binding; ATPase activity; myosin binding

Biological Process: actin filament-based movement; actin-myosin filament sliding; actomyosin structure organization and biogenesis; apoptosis; cardiac muscle morphogensis; cardiac myofibril assembly; heart contraction; muscle filament sliding; muscle thin filament assembly

Disease: Atrial Septal Defect 5; Cardiomyopathy, Dilated, 1r; Cardiomyopathy, Familial Hypertrophic, 11

NCBI Summary:Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]
UniProt Code:P68032
NCBI GenInfo Identifier:54036697
NCBI Gene ID:70
NCBI Accession:P68032.1
UniProt Secondary Accession:P68032,P04270,
UniProt Related Accession:P68032
Molecular Weight:42,019 Da
NCBI Full Name:Actin, alpha cardiac muscle 1
NCBI Synonym Full Names:actin, alpha, cardiac muscle 1
NCBI Official Symbol:ACTC1  
NCBI Official Synonym Symbols:ACTC; ASD5; CMD1R; CMH11; LVNC4  
NCBI Protein Information:actin, alpha cardiac muscle 1
UniProt Protein Name:Actin, alpha cardiac muscle 1
UniProt Synonym Protein Names:Alpha-cardiac actin
Protein Family:Actin
UniProt Gene Name:ACTC1  
UniProt Entry Name:ACTC_HUMAN
View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose