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ACTB Polyclonal Antibody (CABC006)

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SKU:
CABC006
  • Western blot - ACTB Polyclonal Antibody (CABC006)
  • Immunofluorescence - ACTB Polyclonal Antibody (CABC006)
$298.59

Description

Overview

Title:ACTB Polyclonal Antibody (CABC006)
Size:100uL
Code:CABC006
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).
GeneID:60
SwissProt:P60709
Synonyms:ACTB; BRWS1; PS1TP5BP1; beta actin
Calculated MW:41kDa
Observed MW:42kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF;
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:THP-1, 293T, A-431, Mouse lung, Mouse lung, Mouse spleen, Mouse kidney, Mouse thymus
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - ACTB Polyclonal Antibody (CABC006)

Western blot analysis of extracts of various cell lines, using Beta-actin antibody (CABC006) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunofluorescence - ACTB Polyclonal Antibody (CABC006)

Immunofluorescence analysis of HeLa cells using ACTB antibody (CABC006) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Additional Information

ANTIBODY TYPE:
Polyclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Rabbit
ISOTYPE:
IgG
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