The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

ACTB Monoclonal Antibody (CABC004)

(No reviews yet) Write a Review
SKU:
CABC004
  • Western blot - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)
  • Western blot - ACTB Monoclonal Antibody (CABC004)
$298.59

Description

Overview

Title:ACTB Monoclonal Antibody (CABC004)
Size:100uL
Code:CABC004
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen Information

Immunogen:Fusion protein of human ?-actin
GeneID:60
SwissProt:P60709
Synonyms:ACTB; BRWS1; PS1TP5BP1; beta actin
Calculated MW:41kDa
Observed MW:42kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF;
Recommended Dilution:WB 1:10000 - 1:100000; IHC 1:100- 1:200; IF 1:50- 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - ACTB Monoclonal Antibody (CABC004)

Western blot analysis of extracts of various cell lines, using Beta-actin antibody (CABC004) at 1:80000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - ACTB Monoclonal Antibody (CABC004)

Western blot analysis of extracts of NIH/3T3 cells, using Beta-actin antibody (CABC004) at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded rat spleen using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded rat heart using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded human stomach using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded mouse liver using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded mouse spleen using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded mouse heart using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded rat liver using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC004)

Immunohistochemistry of paraffin-embedded rat testis using Beta-actin antibody (CABC004) at dilution of 1:500 (40x lens).
View AllClose

Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Mouse
ISOTYPE:
IgG
View AllClose