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ACSF3 Polyclonal Antibody (CAB13769)

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SKU:
CAB13769
$331.89

Description

Overview

Title:ACSF3 Polyclonal Antibody (CAB13769)
Size:100µL
Code:CAB13769
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human ACSF3 (NP_001230208.1).
GeneID:197322
SwissProt:Q4G176
Synonyms:ACSF3; acyl-CoA synthetase family member 3
Calculated MW:64kDa
Observed MW:64kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, 293T, Mouse liver, Mouse heart, Mouse kidney, Rat brain
Cellular Location:Mitochondrion

Antibody Images

Western blot - ACSF3 Polyclonal Antibody (CAB13769)

Western blot analysis of extracts of various cell lines, using ACSF3 antibody (CAB13769) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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