ACP1 Polyclonal Antibody (CAB1801)

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CAB1801
  • Western blot - ACP1 Polyclonal Antibody (CAB1801)
  • Immunohistochemistry - ACP1 Polyclonal Antibody (CAB1801)
  • Immunohistochemistry - ACP1 Polyclonal Antibody (CAB1801)
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Description

Antibody DatasheetAntibody MSDS

ACP1 Polyclonal Antibody (CAB1801)

Overview

Title:ACP1 Polyclonal Antibody (CAB1801)
Size:100µL
Code:CAB1801
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 15-144 of human ACP1 (NP_004291.1).
GeneID:52
SwissProt:P24666
Synonyms:ACP1; HAAP; LMW-PTP; LMWPTP; acid phosphatase 1, soluble
Calculated MW:12kDa/14kDa/17kDa/18kDa
Observed MW:18kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:Mouse brain, Mouse liver, Mouse skin
Cellular Location:Cytoplasm

Antibody Images

Western blot - ACP1 Polyclonal Antibody (CAB1801)

Western blot analysis of extracts of various cell lines, using ACP1 antibody (CAB1801) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Immunohistochemistry - ACP1 Polyclonal Antibody (CAB1801)

Immunohistochemistry of paraffin-embedded human stomach using ACP1 antibody (CAB1801) at dilution of 1:100 (40x lens).

Immunohistochemistry - ACP1 Polyclonal Antibody (CAB1801)

Immunohistochemistry of paraffin-embedded human liver injury using ACP1 antibody (CAB1801) at dilution of 1:100 (40x lens).

ACP1 Polyclonal Antibody (CAB1801) Protein Information

UniProt Protein Function:ACP1: a phosphotyrosine protein phosphatase. Functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. Hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. Genetically polymorphic with three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.48; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: internal side of plasma membrane; cytoplasm

Molecular Function:protein binding; acid phosphatase activity; non-membrane spanning protein tyrosine phosphatase activity

NCBI Summary:The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:P24666
NCBI GenInfo Identifier:1709543
NCBI Gene ID:52
NCBI Accession:P24666.3
UniProt Related Accession:P24666
Molecular Weight:Molecular Mass: 19.04kDaActual Protein Molecular Mass: 18kDa
NCBI Full Name:Low molecular weight phosphotyrosine protein phosphatase
NCBI Synonym Full Names:acid phosphatase 1
NCBI Official Symbol:ACP1  
NCBI Official Synonym Symbols:HAAP; LMWPTP; LMW-PTP  
NCBI Protein Information:low molecular weight phosphotyrosine protein phosphatase
UniProt Protein Name:Low molecular weight phosphotyrosine protein phosphatase
UniProt Synonym Protein Names:Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase (EC:3.1.3.2); Red cell acid phosphatase 1
Protein Family:Low molecular weight phosphotyrosine protein phosphatase
UniProt Gene Name:ACP1  
UniProt Entry Name:PPAC_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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