ACKR3 Polyclonal Antibody (CAB12712)

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CAB12712
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Description

Antibody DatasheetAntibody MSDS

ACKR3 Polyclonal Antibody (CAB12712)

Overview

Title:ACKR3 Polyclonal Antibody (CAB12712)
Size:100µL
Code:CAB12712
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ACKR3 (NP_064707.1).
GeneID:57007
SwissProt:P25106
Synonyms:ACKR3; CMKOR1; CXC-R7; CXCR-7; CXCR7; GPR159; RDC-1; RDC1; atypical chemokine receptor 3
Calculated MW:41kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Raji, A-549, U-87MG, Mouse lung, Mouse brain, Rat brain
Cellular Location:Cell membrane, Cytoplasm, Early endosome, Multi-pass membrane protein, Recycling endosome, perinuclear region

Antibody Images

Western blot - ACKR3 Polyclonal Antibody (CAB12712)

Western blot analysis of extracts of various cell lines, using ACKR3 antibody (CAB12712) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.

ACKR3 Polyclonal Antibody (CAB12712) Protein Information

UniProt Protein Function:CMKOR1: Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cell surface; coated pit; endosome; intracellular membrane-bound organelle; plasma membrane

Molecular Function:C-X-C chemokine binding; C-X-C chemokine receptor activity; protein binding; scavenger receptor activity

Biological Process: receptor internalization

NCBI Summary:This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]
UniProt Code:P25106
NCBI GenInfo Identifier:115502380
NCBI Gene ID:57007
NCBI Accession:P25106.3
UniProt Secondary Accession:P25106,Q53RV4, Q8NE10, Q92938, Q92986, A8K6J4,
UniProt Related Accession:P25106
Molecular Weight:41,493 Da
NCBI Full Name:Atypical chemokine receptor 3
NCBI Synonym Full Names:atypical chemokine receptor 3
NCBI Official Symbol:ACKR3  
NCBI Official Synonym Symbols:RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159  
NCBI Protein Information:atypical chemokine receptor 3
UniProt Protein Name:Atypical chemokine receptor 3
UniProt Synonym Protein Names:C-X-C chemokine receptor type 7; CXC-R7; CXCR-7; Chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog; RDC-1
Protein Family:Non-reducing polyketide synthase
UniProt Gene Name:ACKR3  
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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