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ACHE Polyclonal Antibody (CAB2806)

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CAB2806
  • Western blot - ACHE Polyclonal Antibody (CAB2806)
  • Immunohistochemistry - ACHE Polyclonal Antibody (CAB2806)
$331.89

Description

Overview

Title:ACHE Polyclonal Antibody (CAB2806)
Size:100µL
Code:CAB2806
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 398-617 of human ACHE (NP_056646.1).
GeneID:43
SwissProt:P22303
Synonyms:ACHE; ACEE; ARACHE; N-ACHE; YT; acetylcholinesterase
Calculated MW:58kDa/65kDa/67kDa
Observed MW:75kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:SH-SY5Y, SGC-996, Mouse brain, Mouse spinal cord, Mouse liver, Rat spinal cord
Cellular Location:Cell junction, Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Nucleus, Peripheral membrane protein, Secreted, synapse

Antibody Images

Western blot - ACHE Polyclonal Antibody (CAB2806)

Western blot analysis of extracts of various cell lines, using ACHE antibody (CAB2806) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - ACHE Polyclonal Antibody (CAB2806)

Immunohistochemistry of paraffin-embedded mouse brain using ACHE antibody (CAB2806) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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