ACAN Polyclonal Antibody (CAB8536)

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CAB8536
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Description

Antibody DatasheetAntibody MSDS

ACAN Polyclonal Antibody (CAB8536)

Overview

Title:ACAN Polyclonal Antibody (CAB8536)
Size:100µL
Code:CAB8536
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human ACAN (NP_001126.3).
GeneID:176
SwissProt:P16112
Synonyms:ACAN; AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK; aggrecan
Calculated MW:239kDa/246kDa/250kDa
Observed MW:100kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HL-60, HepG2, Mouse liver, Mouse brain
Cellular Location:Secreted, extracellular matrix, extracellular space

Antibody Images

Western blot - ACAN Polyclonal Antibody (CAB8536)

Western blot analysis of extracts of various cell lines, using ACAN antibody (CAB8536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

ACAN Polyclonal Antibody (CAB8536) Protein Information

UniProt Protein Function:ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region

Molecular Function:protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process; cell adhesion; proteolysis; skeletal development

Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type

UniProt Code:P16112
NCBI GenInfo Identifier:129886
NCBI Gene ID:
NCBI Accession:P16112.2
Molecular Weight:
NCBI Full Name:Aggrecan core protein
UniProt Protein Name:Aggrecan core protein
UniProt Synonym Protein Names:Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1
UniProt Gene Name:ACAN  
UniProt Entry Name:PGCA_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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