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ACAN Polyclonal Antibody (CAB8536)

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SKU:
CAB8536
$331.89

Description

Overview

Title:ACAN Polyclonal Antibody (CAB8536)
Size:100µL
Code:CAB8536
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human ACAN (NP_001126.3).
GeneID:176
SwissProt:P16112
Synonyms:ACAN; AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK; aggrecan
Calculated MW:239kDa/246kDa/250kDa
Observed MW:100kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HL-60, HepG2, Mouse liver, Mouse brain
Cellular Location:Secreted, extracellular matrix, extracellular space

Antibody Images

Western blot - ACAN Polyclonal Antibody (CAB8536)

Western blot analysis of extracts of various cell lines, using ACAN antibody (CAB8536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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