Description
Title: | ACADM Polyclonal Antibody (CAB1873) | |
Size: | 100µL | |
Code: | CAB1873 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1). |
GeneID: | 34 |
SwissProt: | P11310 |
Synonyms: | ACADM; ACAD1; MCAD; MCADH; acyl-CoA dehydrogenase, C-4 to C-12 straight chain |
Calculated MW: | 46kDa/47kDa |
Observed MW: | 46kDa |
Applications
Reactivity: | Human, Mouse, Rat |
Tested Applications: | WB; IHC; IF |
Recommended Dilution: | WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Application Key: | Western blotting Immunohistochemistry Immunofluorescence |
Positive Samples: | HT-29, HepG2, U-251MG, K562, Mouse liver, Mouse kidney, Mouse brain, Mouse heart, Rat liver |
Cellular Location: | Mitochondrion matrix |
Antibody Images
Western blot analysis of extracts of various cell lines, using ACADM antibody (CAB1873) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. | |
Immunofluorescence analysis of HeLa cells using ACADM antibody (CAB1873) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |