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ACADM Polyclonal Antibody (CAB1873)

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CAB1873
  • Western blot - ACADM Polyclonal Antibody (CAB1873)
  • Immunofluorescence - ACADM Polyclonal Antibody (CAB1873)
$331.89

Description

Overview

Title:ACADM Polyclonal Antibody (CAB1873)
Size:100µL
Code:CAB1873
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1).
GeneID:34
SwissProt:P11310
Synonyms:ACADM; ACAD1; MCAD; MCADH; acyl-CoA dehydrogenase, C-4 to C-12 straight chain
Calculated MW:46kDa/47kDa
Observed MW:46kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HT-29, HepG2, U-251MG, K562, Mouse liver, Mouse kidney, Mouse brain, Mouse heart, Rat liver
Cellular Location:Mitochondrion matrix

Antibody Images

Western blot - ACADM Polyclonal Antibody (CAB1873)

Western blot analysis of extracts of various cell lines, using ACADM antibody (CAB1873) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - ACADM Polyclonal Antibody (CAB1873)

Immunofluorescence analysis of HeLa cells using ACADM antibody (CAB1873) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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