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ABL1 Polyclonal Antibody (CAB0282)

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SKU:
CAB0282
  • Western blot - ABL1 Polyclonal Antibody (CAB0282)
  • Immunohistochemistry - ABL1 Polyclonal Antibody (CAB0282)
  • Immunohistochemistry - ABL1 Polyclonal Antibody (CAB0282)
  • Immunofluorescence - ABL1 Polyclonal Antibody (CAB0282)
$331.89

Description

Overview

Title:ABL1 Polyclonal Antibody (CAB0282)
Size:100µL
Code:CAB0282
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

Immunogen Information

Immunogen:A synthetic peptide of human ABL1
GeneID:25
SwissProt:P00519
Synonyms:ABL1; ABL; JTK7; bcr/abl; c-ABL; c-ABL1; p150; v-abl; tyrosine-protein kinase ABL1
Calculated MW:122kDa/124kDa
Observed MW:139kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:100 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa
Cellular Location:Cytoplasm, Lipid-anchor, Mitochondrion, Nucleus, Nucleus membrane, cytoskeleton

Antibody Images

Western blot - ABL1 Polyclonal Antibody (CAB0282)

Western blot analysis of extracts of HeLa cells, using ABL1 antibody (CAB0282) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - ABL1 Polyclonal Antibody (CAB0282)

Immunohistochemistry of paraffin-embedded human prostate using ABL1 antibody (CAB0282) at dilution of 1:100 (40x lens).

Immunohistochemistry - ABL1 Polyclonal Antibody (CAB0282)

Immunohistochemistry of paraffin-embedded rat kidney using ABL1 antibody (CAB0282) at dilution of 1:100 (40x lens).

Immunofluorescence - ABL1 Polyclonal Antibody (CAB0282)

Immunofluorescence analysis of A431 cells using ABL1 antibody (CAB0282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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