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ABCA4 Polyclonal Antibody (CAB10556)

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CAB10556
  • Western blot - ABCA4 Polyclonal Antibody (CAB10556)
  • Immunofluorescence - ABCA4 Polyclonal Antibody (CAB10556)
$331.89

Description

Overview

Title:ABCA4 Polyclonal Antibody (CAB10556)
Size:100µL
Code:CAB10556
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essental molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macular degeneration. Mutations in this gene are also associated with retinitis pigmentosa-19, cone-rod dystrophy type 3, early-onset severe retinal dystrophy, fundus flavimaculatus, and macular degeneration age-related 2.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 110-300 of human ABCA4 (NP_000341.2).
GeneID:24
SwissProt:P78363
Synonyms:ABCA4; ABC10; ABCR; ARMD2; CORD3; FFM; RMP; RP19; STGD; STGD1; ATP binding cassette subfamily A member 4
Calculated MW:255kDa
Observed MW:256kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:1000 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:U-251MG, BT-474, 293T, Mouse heart, Mouse lung, Rat eye
Cellular Location:Membrane, Multi-pass membrane protein

Antibody Images

Western blot - ABCA4 Polyclonal Antibody (CAB10556)

Western blot analysis of extracts of various cell lines, using ABCA4 antibody (CAB10556) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunofluorescence - ABCA4 Polyclonal Antibody (CAB10556)

Immunofluorescence analysis of HeLa cells using ABCA4 antibody (CAB10556) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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