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ABAT Polyclonal Antibody (CAB5299)

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CAB5299
  • Western blot - ABAT Polyclonal Antibody (CAB5299)
  • Immunohistochemistry - ABAT Polyclonal Antibody (CAB5299)
  • Immunohistochemistry - ABAT Polyclonal Antibody (CAB5299)
  • Immunohistochemistry - ABAT Polyclonal Antibody (CAB5299)
  • Immunofluorescence - ABAT Polyclonal Antibody (CAB5299)
$331.89

Description

Overview

Title:ABAT Polyclonal Antibody (CAB5299)
Size:100µL
Code:CAB5299
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human ABAT (NP_001120920.1).
GeneID:18
SwissProt:P80404
Synonyms:ABAT; GABA-AT; GABAT; NPD009; 4-aminobutyrate aminotransferase
Calculated MW:56kDa
Observed MW:49kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:SW480, A-375, Mouse liver, Mouse kidney, Mouse brain
Cellular Location:Mitochondrion matrix

Antibody Images

Western blot - ABAT Polyclonal Antibody (CAB5299)

Western blot analysis of extracts of various cell lines, using ABAT antibody (CAB5299) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - ABAT Polyclonal Antibody (CAB5299)

Immunohistochemistry of paraffin-embedded rat kidney using ABAT antibody (CAB5299) at dilution of 1:100 (40x lens).

Immunohistochemistry - ABAT Polyclonal Antibody (CAB5299)

Immunohistochemistry of paraffin-embedded human liver injury using ABAT antibody (CAB5299) at dilution of 1:100 (40x lens).

Immunohistochemistry - ABAT Polyclonal Antibody (CAB5299)

Immunohistochemistry of paraffin-embedded human gastric cancer using ABAT antibody (CAB5299) at dilution of 1:100 (40x lens).

Immunofluorescence - ABAT Polyclonal Antibody (CAB5299)

Immunofluorescence analysis of A549 cells using ABAT antibody (CAB5299). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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