The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

AARS Polyclonal Antibody (CAB11846)

(No reviews yet) Write a Review
SKU:
CAB11846
$331.89

Description

Overview

Title:AARS Polyclonal Antibody (CAB11846)
Size:100µL
Code:CAB11846
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

Immunogen Information

Immunogen:A synthetic peptide of human AARS
GeneID:16
SwissProt:P49588
Synonyms:AARS; CMT2N; EIEE29; alanyl-tRNA synthetase
Calculated MW:106kDa/109kDa
Observed MW:120kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:K-562, 293T, HeLa, HepG2
Cellular Location:Cytoplasm

Antibody Images

Western blot - AARS Polyclonal Antibody (CAB11846)

Western blot analysis of extracts of various cell lines, using AARS antibody (CAB11846) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
View AllClose