The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

AAAS Polyclonal Antibody (CAB6427)

(No reviews yet) Write a Review
SKU:
CAB6427
  • Western blot - AAAS Polyclonal Antibody (CAB6427)
  • Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)
  • Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)
  • Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)
  • Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)
  • Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)
  • Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)
  • Immunofluorescence - AAAS Polyclonal Antibody (CAB6427)
£254.15

Description

Overview

Title:AAAS Polyclonal Antibody (CAB6427)
Size:100µL
Code:CAB6427
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 287-546 of human AAAS (NP_056480.1).
GeneID:8086
SwissProt:Q9NRG9
Synonyms:AAAS; AAA; AAASb; ADRACALA; ADRACALIN; ALADIN; GL003; aladin
Calculated MW:55kDa/59kDa
Observed MW:60kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:MCF-7, HeLa, 293T
Cellular Location:Nucleus, nuclear pore complex

Antibody Images

Western blot - AAAS Polyclonal Antibody (CAB6427)

Western blot analysis of extracts of various cell lines, using AAAS antibody (CAB6427) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)

Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (CAB6427) at dilution of 1:100 (40x lens).

Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)

Immunohistochemistry of paraffin-embedded rat brain using AAAS Antibody (CAB6427) at dilution of 1:100 (20x lens).

Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)

Immunohistochemistry of paraffin-embedded human appendicitis using AAAS Antibody (CAB6427) at dilution of 1:100 (40x lens).

Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)

Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (CAB6427) at dilution of 1:100 (40x lens).

Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)

Immunohistochemistry of paraffin-embedded mouse brain using AAAS Antibody (CAB6427) at dilution of 1:100 (40x lens).

Immunohistochemistry - AAAS Polyclonal Antibody (CAB6427)

Immunohistochemistry of paraffin-embedded mouse heart using AAAS Antibody (CAB6427) at dilution of 1:100 (40x lens).

Immunofluorescence - AAAS Polyclonal Antibody (CAB6427)

Immunofluorescence analysis of HeLa cells using AAAS antibody (CAB6427). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose