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A4GNT Polyclonal Antibody (CAB12366)

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SKU:
CAB12366
$331.89

Description

Overview

Title:A4GNT Polyclonal Antibody (CAB12366)
Size:100µL
Code:CAB12366
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human A4GNT (NP_057245.1).
GeneID:51146
SwissProt:Q9UNA3
Synonyms:A4GNT; alpha4GnT; alpha-1, 4-N-acetylglucosaminyltransferase
Calculated MW:39kDa
Observed MW:49kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:SGC-7901, 293T, LO2
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein

Antibody Images

Western blot - A4GNT Polyclonal Antibody (CAB12366)

Western blot analysis of extracts of various cell lines, using A4GNT antibody (CAB12366) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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