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A4GALT Polyclonal Antibody (CAB8817)

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SKU:
CAB8817
$331.89

Description

Overview

Title:A4GALT Polyclonal Antibody (CAB8817)
Size:100µL
Code:CAB8817
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. This protein, a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. Alternatively spliced transcript variants have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 244-353 of human A4GALT (NP_059132.1).
GeneID:53947
SwissProt:Q9NPC4
Synonyms:A4GALT; A14GALT; A4GALT1; Gb3S; P(k); P1; P1PK; PK; lactosylceramide 4-alpha-galactosyltransferase
Calculated MW:40kDa
Observed MW:45kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:293T, LO2, A-375, HeLa, Mouse kidney, Mouse brain, Rat kidney
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein

Antibody Images

Western blot - A4GALT Polyclonal Antibody (CAB8817)

Western blot analysis of extracts of various cell lines, using A4GALT antibody (CAB8817) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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