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A1CF Polyclonal Antibody (CAB13087)

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SKU:
CAB13087
$331.89

Description

Overview

Title:A1CF Polyclonal Antibody (CAB13087)
Size:100µL
Code:CAB13087
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human A1CF (NP_055391.2).
GeneID:29974
SwissProt:Q9NQ94
Synonyms:A1CF; ACF; ACF64; ACF65; APOBEC1CF; ASP; APOBEC1 complementation factor
Calculated MW:55kDa/58kDa/62kDa/64kDa/65kDa
Observed MW:65kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, BxPC-3, HT-29, HeLa, Mouse liver
Cellular Location:Cytoplasm, Endoplasmic reticulum, Nucleus

Antibody Images

Western blot - A1CF Polyclonal Antibody (CAB13087)

Western blot analysis of extracts of various cell lines, using A1CF antibody (CAB13087) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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